Part:BBa_K4825017

ATF1

ATF1 is derived from S.cerevisiae genome. It encodes for the enzyme alcohol acetyltransferase 1 (AATase 1), and catalyses the reaction of acetate esters from acetyl-CoA and alcohols. It is included in our composite part with ARO10 (2-ketoacid decarboxylase) and ADH7 (alcohol dehydrogenase), which are transformed into the genome of S.cerevisiae simultaneously for overexpression to produce isoamyl acetate.

Usage and Biology

ATF1 is a DNA sequence derived from the genome of S.cerevisiae that encodes for the enzyme alcohol acetyltransferase 1 (AATase-1). It catalyzes the conversion from acetyl-CoA and alcohols to acetate esters. During fermentation, the rates of producing acetate esters demonstrate a correlation to AATase activity.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 412
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 412
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 412
  Illegal BglII site found at 99
  Illegal BamHI site found at 1426
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 412
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 412
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 526
  Illegal SapI.rc site found at 1534